Effect of RNA Shelf Life on Cycle Threshold (CT) Values in Patients Positively SARS CoV-2 with RRT-PCR Method
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Abstract
Introduction: The shelf life of RNA is one of the things to consider in the success of the rRT-PCR test. RNA storage that is not properly monitored can cause unstable Cycle Threshold (CT) results. The conditions for storing RNA are 4 °C if the RNA will be used directly for PCR, -20 °C if it will be used within 24 hours.
Objective: The purpose of this study was to determine the effect of the RNA storage period at -20 °C on the Cycle Threshold value in Sars-CoV-2 positive patients using the rRT-PCR method after the RNA storage period was carried out.
Methods: This study was experimental with 3 treatment including 1 control and 2 treatments of RNA storage (24 h and 48 h). The results of the amplification stage show no difference in the results of the Cycle Threshold value between the control and the RNA storage life.
Results: These results were obtained from Kruskal Wallis statistical test with significant value 0,996 for target gene of E and 0,976 for target gene of RdRp.
Conclusions: From the results of this study, it can be concluded that the RNA storage life of 24 hours and 48 hours at -20 °C does not effect the Cycle Threshold values in Sars-CoV-2 positive patients using the rRT-PCR method
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